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KMID : 0380020060210040302
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 4 p.302 ~ p.305
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Rapid Preparation of Truncated Transaminases using a PCR-based Cell-free Protein Synthesis System
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Kwon Yong-Chan
Park Kyung-Moon
Kim Dong-Myung
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Abstract
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In this work, we attempted the application of cell-free protein synthesis technology for the rapid generation of truncated enzymes. Truncated DNAs of a transaminase were PCR-amplified and directly expressed in cell-free protein synthesis reactions. Variants of the transaminase were rapidly prepared and analyzed for their enzymatic activity. Described method that combines the PCR and cell-free protein synthesis technologies will offer a versatile platform for the rapid generation of optimally modified protein species.
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KEYWORD
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